# Single-Cell Sequencing: Experimental Design, Analysis, and Practical Applications Semester: Spring | Year offered: 2019 Single-cell sequencing technologies allow us to study genomes and transcriptomes at the single cell level. These relatively new techniques have expanded our knowledge in a wide range of fields from microbiology to development and cancer biology. This course will provide a general introduction to both RNA and DNA-based single-cell sequencing. We will start with a broad overview of RNA-sequencing in single cells with an emphasis on current and emerging technologies. Subsequent lectures will focus on experimental design for single-cell experiments and common approaches to data analysis. Class Assignments Registered participants will use the technologies, experimental design, and analysis methods presented in Session 1 to design a single-cell sequencing experiment. They will write a one-page specific aims proposal and give a 5-minute presentation of the experiment. Course Instructors: Evan Macosko, and Mandovi Chatterjee,[Mandovi\_Chatterjee@hms.harvard.edu](mailto:Mandovi_Chatterjee@hms.harvard.edu) Course Director: Peter Kharchenko, [Peter\_Kharchenko@hms.harvard.edu](mailto:///Peter_Kharchenko@hms.harvard.edu) Curriculum Fellow: Rachel Wright, [rachel\_wright@hms.harvard.edu](mailto:///rachel_wright@hms.harvard.edu) Session Dates and Times First Session Date: March 7, 2019 - 1:00pm - 4pm First Session Location:TMEC 227 Second Session Date: March 14, 2019 - 1:00pm - 4pm Second Session Location: For Registered participants only Peter's analysis walkthrough: ### [**CLICK HERE to register for this course.**](https://docs.google.com/forms/d/e/1FAIpQLScW0N2CzkgY7siyvAYOZteEaIW2dHOSmetfDdOrd4bZQoxIXw/viewform?usp=sf_link)