Microscopy Measurements: Considerations for accurate and precise quantitative microscopy





Course Description

Fluorescence microscopy can be a very powerful tool for making quantitative spatial and intensity measurements . However, sources of error and imprecision are inherent in every modality of light microscopy and digital image acquisition, and can lead to inaccurate conclusions if left unaddressed. In this nanocourse, we will discuss common sources of inaccuracy and imprecision in quantitative microscopy, and how to identify and address them. In the virtual laboratory exercises, you will learn how to estimate signal-to-noise ratio and resolution in images, and how these important determinants of image quality relate to one another.

Course Expectations

The course is comprised of two lecture sessions and a discussion section (see details below).  Attendance at all sessions is mandatory.  You will be expected to read an assigned paper, complete an image analysis exercise, and be prepared to discuss the paper and execise at the Friday discussion session.


Session Details and Dates

This course will be live-streamed over Zoom. It is free and open to all, but participants must register below to receive the Zoom link. Attendance at all sessions is mandatory.

Session 1: Monday, June 1st,  10:30am - 12:00pm

Session 2: Wednesday, June 3rd, 10:30am-12:00pm

Discussion: Friday, June 5th, 1:00pm-4:00pm 



Jennifer C. Waters, PhD (Director of the Nikon Imaging Center & Lecturer in Cell Biology, Harvard Medical School)

Talley Lambert, PhD (Lecturer on Systems Biology)


Curriculum Fellow

Brittany Michel (brittany_michel@hms.harvard.edu)


Register here to get access to the Zoom sessions.